Figure 3.

Colocalization of AtSLP1 with fluorescent marker constructs in fava bean leaves. A and B show representative images of guard cells cobombarded with AtSLP1-RFP (red) and either ER- or peroxisome-targeted GFP (green), respectively. Chlorophyll autofluorescence (blue) is shown along with the merged image (right). The specific organelle targeting of GFP was achieved though fusion with conserved targeting motifs (Nelson et al., 2007). All images are single slices obtained using a confocal laser scanning microscope (Leica). Bars = 10 μm.