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. 2011 Oct 5;157(4):1778–1792. doi: 10.1104/pp.111.182493

Figure 5.

Figure 5.

Spatial and temporal western-blot analysis of AtSLP1 and AtSLP2 protein expression. A, Tissue-specific expression of AtSLP1 and AtSLP2 from different Arabidopsis Columbia tissues. Each lane contains 30 μg of clarified cell lysate. B, Time-course analysis of AtSLP1 protein expression over a 24-h light/dark cycle. Each lane contains 30 μg of clarified lysate from rosette leaf tissue. All tissues were harvested from 21-d-old plants. The bottom panels of both A and B represent Ponceau S (0.1%, w/v)-stained membranes demonstrating equal protein loading prior to incubation with either affinity-purified (AP) anti-AtSLP1 or anti-AtSLP2 IgG.