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. 2011 Oct 5;157(4):1778–1792. doi: 10.1104/pp.111.182493

Figure 6.

Figure 6.

Primary amino acid alignment of AtSLP1 and AtSLP2 full-length sequences with representative PP1 sequences from Arabidopsis (TOPP2 [At5g59160] and AtPP2A-1 [At1g59830]) and human (HsPP1γ; NP_002701). Alignment was performed using ClustalX followed by import into GeneDoc for image assembly. Solid lines represent highly conserved motifs comprising the active site of canonical Ser/Thr phosphatases. Dashed lines represent a highly conserved region in PPPs that conveys sensitivity to MC-LR through its covalent attachment to Cys-273 (HsPP1γ) and an equivalent residue in a plant PP1, Cys-279 (TOPP2; section mark). The amino acids marked by black circles represent those specifically important to MC-LR binding, while those marked by black squares are specific to OA binding but are also key in coordinating MC-LR. The arrows denote amino acids found to play a role in metal ion coordination, while the black triangles denote amino acids found to dock the PP1 I-2 protein. Amino acids denoted with white squares have been implicated in the binding of MC-LR, OA, and I-2.