Figure 9.

Assessment of purified, untagged AtSLP1 sensitivity to small molecule inhibitors. A and B, Inhibition curves comparing the phosphatase inhibitor sensitivity of AtSLP1 (black triangles) with TOPP2 (white circles) and HsPP1γ (black circles) using the potent PPP inhibitors OA and MC. C to E, Inhibition curves comparing the PPi, Pi, and NaF sensitivity of AtSLP1 with TOPP2. All phosphatase assays were conducted using the small molecule substrate pNPP as outlined in “Materials and Methods.”