Fig. 5.

Distribution of velocities for Myo6 in the absence and presence of plus-end myosins at Myo2 (A) and Myo5a (B) concentrations that exhibit maximum increases in Myo6 motility. Gliding velocities as a function of number of filaments observed by 0.014 μM Myo6 in the absence (open bars) or presence (hatched bars) of either 0.5 nM Myo2 (A) or 0.9 nM Myo5a (B) are shown. Note that there is no overlap in velocities in the absence and presence of plus-end motor, indicating that the increased velocities is not an average of slower Myo6-minus end movement and faster plus-end motor mediated movement.