Table 2.
Robotic Pick-and-Place (P&P) Performance on 6–h Postfertilization (hpf) Dechorionated Zebrafish Embryos Compared to Manual Plate Loading
| Overall P&P Performance |
Specific P&P Failures |
||||||
|---|---|---|---|---|---|---|---|
| Trial Date | Wells | Wells Allocated Successfully, No. (%)a |
Wells Unsuccessful, No. (%)b |
P&P Mortalityc |
Missed Wellsd |
Multipickse | Malformedf |
| 8/1/2011 | 96 | 86 (89.6) | 10 (10.4) | 2 | 5 | 0 | 3 |
| 8/2/2011 | 96 | 90 (93.8) | 6 (6.3) | 4 | 0 | 0 | 2 |
| 8/2/2011 | 96 | 93 (96.9) | 3 (3.1) | 2 | 0 | 0 | 1 |
| 8/2/2011 | 96 | 95 (99.0) | 1 (1.0) | 0 | 0 | 0 | 1 |
| 8/4/2011 | 96 | 85 (88.5) | 11 (11.5) | 9 | 0 | 0 | 2 |
| 8/4/2011 | 96 | 91 (94.8) | 5 (5.2) | 2 | 1 | 0 | 2 |
| 8/5/2011 | 96 | 88 (91.7) | 8 (8.3) | 6 | 0 | 0 | 2 |
| 8/8/2011 | 96 | 87 (90.6) | 9 (9.4) | 6 | 0 | 1 | 2 |
| 8/9/2011 | 96 | 89 (92.7) | 7 (7.3) | 4 | 2 | 0 | 1 |
| 9/6/2011 | 96 | 95 (99.0) | 1 (1.0) | 0 | 1 | 0 | — |
| 9/6/2011 | 96 | 91 (94.8) | 5 (5.2) | 0 | 5 | 1 | — |
| 9/6/2011 | 96 | 96 (100.0) | 0 | 0 | 0 | 0 | — |
| 9/7/2011 | 96 | 84 (87.5) | 12 (12.5) | 8 | 1 | 3 | — |
| 9/7/2011 | 96 | 95 (99.0) | 1 (1.0) | 0 | 1 | 0 | — |
| 9/7/2011 | 96 | 95 (99.0) | 1 (1.0) | 0 | 1 | 0 | — |
| 9/7/2011 | 96 | 95 (99.0) | 1 (1.0) | 0 | 1 | 0 | — |
| Ave = 94.7% ± 4.2%g | 2.8% | 1.2% | 0.3% | 1.9% | |||
| n = 16 trials | |||||||
|
| |||||||
| Overall Manual Performance |
Specific Manual Failures |
||||||
| Trial Date | Wells | Wells Allocated Successfully, No. (%) |
Wells Unsuccessful, No. (%) |
Manual Load Mortality |
Missed Wells |
Multipicks | Malformed |
|
| |||||||
| 8/1/2011h | 96 | 87 (90.6) | 9 (9.4) | 6 | 1 | 1 | 1 |
| 8/2/2011h | 96 | 89 (92.7) | 7 (7.3) | 7 | 0 | 0 | 0 |
| 8/2/2011h | 96 | 87 (90.6) | 9 (9.4) | 8 | 0 | 0 | 1 |
| 8/2/2011h | 96 | 91 (94.8) | 5 (5.2) | 1 | 0 | 1 | 3 |
| 8/4/2011h | 96 | 86 (89.6) | 10 (10.4) | 10 | 0 | 0 | 0 |
| 8/4/2011 | 96 | 91 (94.8) | 5 (5.2) | 5 | 0 | 0 | 0 |
| 8/5/2011 | 96 | 91 (94.8) | 5 (5.2) | 3 | 0 | 0 | 2 |
| 8/8/2011 | 96 | 88 (91.7) | 8 (8.3) | 6 | 0 | 0 | 2 |
| 8/9/2011 | 96 | 96 (100.0) | 0 | 0 | 0 | 0 | 0 |
| 9/6/2011 | 96 | 96 (100.0) | 0 | 0 | 0 | 0 | — |
| 9/6/2011 | 96 | 96 (100.0) | 0 | 0 | 0 | 0 | — |
| 9/6/2011 | 96 | 89 (92.7) | 7 (7.3) | 7 | 0 | 0 | — |
| 9/7/2011 | 96 | 89 (92.7) | 7 (7.3) | 7 | 0 | 0 | — |
| 9/7/2011 | 96 | 96 (100.0) | 0 | 0 | 0 | 0 | — |
| 9/7/2011 | 96 | 94 (97.9) | 2 (2.1) | 1 | 0 | 1 | — |
| 9/7/2011 | 96 | 95 (99.0) | 1 (1.0) | 1 | 0 | 0 | — |
| Ave = 94.9% ± 3.6% | 4.0% | — | 0.2% | 1.0% | |||
| n = 16 trials | |||||||
Well allocation is placement of one dechorionated 6-hpf embryo.
An unsuccessful well allocation (errorsc-e) was determined immediately after the trial and at 24 hpf for.
Embryo dead, usually partly or completely disintegrated, immediately after trial.
Embryo or disintegrated residue absent from well immediately after trial.
More than one embryo allocated to the same well.
Malformations observed at 24 hpf; Dash indicates not recorded 9/6–9/7.
Robotic performance was not different from the overall success of manual loading (F = 0.53; p < 0.01).
Lower apparent performance success of earlier trials reflects the performance of earlier software versions.