Fig. 1.

Confirmation of KAR2 conditional null mutant in Candida albicans through Southern blot analysis. The diagram on the left depicts the three expected restriction fragments after the digestion of genomic DNAs with AlwNI. When probed, BWP17 (lane 1) results in a single band, representing two wild-type KAR2 alleles. Placing one KAR2 allele under control of the MET3 promoter (BPY104; lane 2) increases the size of the restriction fragment of one allele so that two bands are evident. The UAU1 disruption of the remaining wild-type KAR2 allele (BPY116; lane 3) corresponds to the emergence of the largest restriction fragment, the continued presence of the MET3 controlled allele, and the disappearance of the band corresponding to the wild-type size