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. Author manuscript; available in PMC: 2012 Apr 16.
Published in final edited form as: Curr Genet. 2010 Oct 1;57(1):25–37. doi: 10.1007/s00294-010-0323-1

Fig. 2.

Fig. 2

The Candida albicans KAR2 gene is essential. Strains BWP17 (KAR2/KAR2), BPY104 (MET3p-KAR2/KAR2), BPY116a, and BPY116b (both MET3p-KAR2/kar2::UAU1) were streaked onto plates under conditions in which the MET3p-controlled KAR2 gene was either induced in medium lacking methionine and cysteine (a) or repressed in medium containing 2.5 mM methionine and 2.5 mM cysteine (b). Both plates were grown at 30°C for ~48 h. The conditional null mutants (BPY116a and BPY116b) are inviable when KAR2 expression is repressed by the MET3 promoter (b)