Fig. 3.

The C. albicans KAR2 gene can rescue a temperature-sensitive allele of the S. cerevisiae kar2 gene. A S. cerevisiae strain containing the kar2-159 mutation (RSY586) was transformed with a plasmid expressing the C. albicans KAR2 gene, generating strain MMY102. Liquid cultures of these cells were grown in appropriate media overnight along with a wild-type S. cerevisiae strain (RSY607) and strain MMY101, which is RSY586 containing the empty vector. The cultures were normalized to a common OD₆₀₀, and 5 µl of each culture (1) and tenfold serial dilutions (2–4) were then spotted onto two YPD plates. One plate was incubated at 22°C and the other at 37°C for ~48 h