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. 2012 Apr 15;23(8):1524–1532. doi: 10.1091/mbc.E11-12-0999

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© 2012 Roth et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Germline stem cell centrosome orientation is modulated by nutrient availability. (a) Schematic diagram of centrosome movement during the GSC cell cycle. (b) Centrosome misorientation frequencies in GSCs from flies cultured in rich or poor media. Numerical data are presented as the mean ± SD in all figures. n > 300/data point. Flies were cultured in the indicated media until the adult stage and then kept in/transferred to the indicated media at day 0. (c) An example of an apical testis tip from a fly cultured in poor media. Although many interphase GSCs (white circles) are misoriented, the mitotic spindle is correctly oriented (yellow circle). Blue, Vasa (germ cells); green, phosphorylated histone H3 (pH3, mitotic chromosomes); red, γ-tubulin (centrosomes). The hub is marked by an asterisk. Scale bar, 10 μm. (d) Bromodeoxyuridine incorporation in GSCs under poor or rich media conditions. n > 100/data point.