Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 15;23(8):1524–1532. doi: 10.1091/mbc.E11-12-0999

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Roth et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Nutrient-poor media do not increase dedifferentiation of germline cells. (a) An example of an apical testis tip with a dedifferentiated GSC, as observed by LacZ expression. LacZ expression was induced by Bam-gal4/UAS-FLP–mediated activation of the LacZ gene, thus marking cells that had once committed to differentiation (Cheng et al., 2008). Green, Vasa (germ cells); red, LacZ; blue, 4′,6-diamidino-2-phenylindole. Hub, asterisk. Scale bar, 10 μm. (b) Frequency of LacZ-positive GSCs in rich and poor media (mean ± SD). (c) An example of apical testes tip containing dedifferentiating GSCs and spermatogonia that are still connected to one another. Green, Pavarotti-GFP (contractile ring and ring canal); red, adducin-like (spectrosome/fusome); blue, Vasa. (d) Frequency of dedifferentiation in rich and poor media (mean ± SD). Flies raised in rich media were transferred to new vials containing either rich or poor media, and testes were examined 2 or 3 d later. (e) Dedifferentiated GSCs do not recover proper centrosome orientation after a prolonged time period. Young adult hs-Bam flies (day 0) were subjected to five heat shocks (30 min each) over the course of 2 d (Sheng et al., 2009) and then cultured at 25°C. GSC centrosome orientation was scored at the indicated time.