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. 2012 Apr 15;23(8):1524–1532. doi: 10.1091/mbc.E11-12-0999

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© 2012 Roth et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Apc2 localization is regulated by the insulin signaling pathway. (a–d) Examples of Apc2 staining in apical testes tips from flies that express a dominant-negative (K1409A) or constitutively active (A1325D) form of InR in rich vs. poor media. Cortical localization of Apc2 is indicated with yellow lines. Cytoplasmic punctae of Apc2 are indicated with yellow arrowheads. Green, Apc2; blue, Vasa (germ cells). (e) Quantification of Apc2 localization upon overexpression of a dominant-negative (K1409A) or constitutively active (A1325D) form of InR, Akt-RNAi, or Akt-HA in rich or poor media (mean ± SD). n > 100 testes/data point.