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. 2012 Apr 15;23(8):1546–1557. doi: 10.1091/mbc.E11-09-0821

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© 2012 Xu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Measurements of ceramide in bovine CAECs by flow cytometry. (A) Frequency histogram of ceramide in control and FasL-treated cells exposed to pH 5.5, 6.0, 6.5, 7.0, and 7.5. (B) The percentage of ceramide positive-staining cells increased due to FasL treatment under different pH conditions. At pH 6.0, the percentage reached the maximum (n = 6, *p < 0.05 vs. control). (C) Top, no significant change of forward and side scatter under different pH conditions, which suggests that the treatment has no effect on cell size or granularity. Bottom, cell viability measured by flow cytometry. Cells were stained by ViaCount reagent. Viable cells appear at the bottom and dead cells at the top. The results show that the viability was not <95% under different pH conditions.