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. 2012 Apr 15;23(8):1546–1557. doi: 10.1091/mbc.E11-09-0821

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© 2012 Xu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Measurement of ceramide concentration by liquid chromatography electrospray ionization tandem mass spectrometry. (A) Ceramide concentration in CAECs was increased due to FasL treatment under different pH conditions. Moreover, ceramide reached a maximum at pH 6.0. (B) ASM siRNA transfection significantly blocked the FasL-induced increase in ceramide production. (n = 4, *p < 0.05 vs. control; ^#p < 0.05 vs. only FasL-treated group).