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. 2012 Apr 15;23(8):1546–1557. doi: 10.1091/mbc.E11-09-0821

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© 2012 Xu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Measurements of H⁺-ATPase translocation in bovine CAECs with flow cytometry. (A) Frequency histogram of H⁺-ATPase in the membranes of control or FasL-stimulated (10 ng/ml for 15 min) cells without or with TT (10 nM) or vacuolin-1 (10 μM) and the overlay. (B) The percentage of H⁺-ATPase positive–staining cells significantly increased in FasL-treated CAECs, which was suppressed by preincubation with vacuolin-1 or TT (n = 6, *p< 0.05 vs. control; ^#p < 0.05 vs. only FasL-treated group).