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. 2012 Apr 15;23(8):1546–1557. doi: 10.1091/mbc.E11-09-0821

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© 2012 Xu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — FRET analysis of the MR marker ganglioside GM1, H⁺-ATPase, and ASM in bovine CAECs. FRET was detected using an acceptor-bleaching protocol. The blue images (representing FRET) on the bottom were obtained by subtracting a prebleaching image from a postbleaching image. (A) Representative images of FRET between V1 H⁺-ATPase and GM1 (CtxB labeling) or ASM. (B) Summarized results of detected FRET efficiency show that FasL significantly increased the FRET efficiency between V1 H⁺-ATPase and GM1 or ASM, which was effectively inhibited by vacuolin-1(10 μM) or V1 H⁺-ATPase siRNA (n = 6, *p < 0.05 vs. control; ^#p < 0.05 vs. only FasL-treated group).