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. 2012 Apr 15;23(8):1593–1604. doi: 10.1091/mbc.E11-11-0900

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© 2012 Oinuma et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Role of the N-terminal sequence of Rnd1 in p190 RhoGAP-mediated RhoA inhibition. (A) Whole-cell lysates from untransfected or GFP-p190 RhoGAP–transfected cells were analyzed by immunoblotting with anti–p190 RhoGAP and anti–α-tubulin antibodies. (B–E) COS-7 cells were transfected with the indicated expression plasmids, and relative RhoA activity was measured as described in the legend to Figure 4, C and D. The results are the mean ± SEM of three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05 (one-way ANOVA).