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. 2012 Apr 15;23(8):1414–1422. doi: 10.1091/mbc.E11-06-0545

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© 2012 Fellows et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Effects of overexpressing AUF1 on endogenous VEGF protein levels. Cultured RAW-264.7 cells were grown to 50% confluence and transfected with full-length AUF1 (AUF1) or the empty parental vector pcDNA-3.1 (PC). After 4 h, cells were treated with LPS for 18 h. Lysates were probed for VEGF and α-tubulin. Two of six experiments are shown. For six experiments, VEGF protein (normalized to α-tubulin) was decreased by 22% (p < 0.04).