FIGURE 2:

Effects of the 3′ UTR of VEGF mRNA on reporter activity in multiple cell types. (A) VEGF-3′ UTR-luciferase (VEGF-3′ UTR-Luc) or the empty parental vector (3.1-Luc) were transfected into RAW-264.7 cells using Lipofectamine 2000. Luciferase activity was measured in cell lysates. (B) Bone marrow–derived macrophages were obtained from C57/Bl6 mouse femurs and cultured in L-400 media. After 6 d, cells were trypsinized and transfected with VEGF-3′ UTR-Luc or 3.1-Luc by electroporation (Amaxa). (C) Normal rat kidney cells (NRK-52E) were cultured for <30 passages and transfected with VEGF-3′ UTR-Luc or 3.1-Luc using Lipofectamine 2000. (D) HEK-293 cells were transfected with VEGF-3′ UTR-Luc or 3.1-Luc using Lipofectamine 2000. All transfections (A–D) were incubated for 18–24 h before cell lysis. Results are normalized to 3.1-Luc transfection, and are representative of two or more experiments. Shown are mean and SD. p < 0.001 for A and C. p < 0.01 for B and D.