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. 2012 Apr 15;23(8):1414–1422. doi: 10.1091/mbc.E11-06-0545

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© 2012 Fellows et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Immunoprecipitation of mRNA associated with AUF1. (A) AUF1 protein was isolated with anti-AUF1 antibody and protein A beads and the pulldown analyzed for AUF1 protein. Control pulldowns were performed with no anti-AUF1 antibody (No Ab). (B) To identify mRNA associated with AUF1, RNA was purified from pulldown beads and the cDNA amplified for VEGF, GLUT1, HK2, or 18S RNA. PCR products are shown.