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. 2012 Apr 15;23(8):1414–1422. doi: 10.1091/mbc.E11-06-0545

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© 2012 Fellows et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Effects of cycloheximide inhibition on V5-tagged protein synthesis. Cultured RAW-264.7 cells were transfected with the full-length AUF1-V5 protein (AUF), the N-terminal deletant AUF1-d21-Ala (d21-Ala), the C-terminal deletant (dQRGG), or the double deletant (d21-Ala+dQRGG). After 24 h, cells were treated with cycloheximide for 0, 1, or 4 h. Cell lysates were immunoblotted with anti-V5 antibody, and protein levels were normalized to α-tubulin levels. Levels of each protein type are expressed relative to untreated controls. The change in deletant protein levels was not significantly different from AUF1 at any time point. Compared to AUF1, the double-deletant protein levels were decreased by 40% after 4 h, but the decrease was not significant (p < 0.10). Results are the mean and SD for three separate experiments.