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. 2012 Apr 15;23(8):1414–1422. doi: 10.1091/mbc.E11-06-0545

FIGURE 9:

FIGURE 9:

Effects of adenosine dialdehyde on AUF1 protein. (A) Cells (RAW-264.7) were treated with AdOx (5 µM) for 48 h, and lysates were immunoblotted for AUF1 isoforms. Loading control was α-tubulin. (B) The effects of AdOx (5 μM for 48 h) were measured on RAW-264.7 cells transfected with AUF1 (WT) or AUF1-ΔQRGG-V5 (dQRGG). Triplicate samples were immunoblotted with anti-V5 antibody. WT, dimethyl sulfoxide (DMSO) vs. AdOx, p < 0.0001; AUF1-ΔQRGG, DMSO vs. AdOx, p < 0.001. Results are normalized to DMSO treatment and are representative of two or more experiments. Shown are mean and SD.