Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 15;23(8):1475–1485. doi: 10.1091/mbc.E11-11-0957

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Frijns et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — EGF and PMA stimulate phosphorylation of β4 at T1736 in PA-JEB/β4 keratinocytes and A431 epidermoid carcinoma cells. PA-JEB/β4 (left) and A431 (right) cells were deprived of growth factors overnight and left untreated or were stimulated with 100 ng/ml PMA or 50 ng/ml EGF for 15 min. The cells were lysed in RIPA buffer and analyzed for the phosphorylation of β4 at T1736, S1356, and S1364, PKD1 (S744/748 or S916), p44/p42 (T202/Y204), and PKC (βII S660) and for total levels of β4 using immunoblotting.