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. 2012 Apr 15;23(8):1446–1456. doi: 10.1091/mbc.E11-09-0797

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© 2012 Wang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — NSN1 represses AG expression in inflorescence meristem and floral meristem. (A–C) In situ RNA hybridization of AG mRNA expression in the floral primordia of wild-type (A) and nsn1/+ plants (B), as well as in the terminal carpelloid floral primordium of homozygous nsn1 plant (C). (D–F) GUS staining of plants harboring KB9 in the control (D) and nsn1 genetic background (E, F). GUS activity was elevated in the inflorescence meristem (IM) and terminal carpelloid flower of nsn1 (F) as compared with the staining in the control plant (D). Ca, carpel-like structure; O, ectopic ovule; St, stamen-like structure. (G–I) A flower of the nsn1 ag double mutant (I) was morphologically identical to that of ag (H) but distinct from that of nsn1-1 (G). The reduced flower size of the double mutant was a result of the difference in ecotype (ag in Landsberg and nsn1 in Columbia-0). Note that the terminal carpelloid flower phenotype of nsn1 was suppressed in the double mutant. CB, carpelloid bract; P, petal. Scale bars, 50 μm (A–F), 1 mm (G–I).