Fig. 7.

Effects of ECE-1 inhibition on resensitization of CRF₁. A, In HEK-CRF₁ cells SM-19712 treatment had no effect on Ca²⁺ signals compared with vehicle upon Ucn1 or CRF (100 nm) stimulation. B, In the presence of SM-19712, both Ucn1 and CRF induced similar desensitization after 0 h of recovery. After 1 h or 4 h recovery, CRF-stimulated Ca²⁺ signals resensitized, whereas Ucn1-stimulated Ca²⁺ signals were inhibited by SM-19712. C, In HEK-CRF₁ cells, SM-19712 treatment had no effect on 30 nm CRF-stimulated Ca²⁺ signals, but did reduce the 30 nm Ucn1-stimulated Ca²⁺ signals. D, At a CRF or Ucn1 concentration of 30 nm, SM-19712 treatment reduced both Ucn1 and CRF-mediated resensitization measured after 1 h recovery (n ≥ 3 experiments; *, P < 0.05 compared with vehicle). Recov., Recovery; Veh., vehicle.