Fig. 5.

Methylation inhibits AR recruitment and Pp-dependent transcription. A, Luciferase analysis of epididymal cells transiently transfected with mock and methylated Pp construct, performed as described in the legend of Fig. 1B. Cells incubated with the synthetic testosterone R1881 (T) and cotransfected with AR expression vector are indicated. B, ChIP analysis of DC2 cells transiently transfected with mock and methylated Pp construct using AR and a negative control antiserum. The values shown are mean (±se) obtained by real-time PCR from three samples run in triplicate. An asterisk indicates statistically significant differences from the control (P ≤ 0.05).