Skip to main content
. Author manuscript; available in PMC: 2012 Apr 16.
Published in final edited form as: J Biol Chem. 2005 Jan 4;280(11):10234–10243. doi: 10.1074/jbc.M411102200

Figure 3. Effect of 12G10 on focal adhesion and actin stress fibre formation in A375-SM cells.

Figure 3

A375-SM cells were spread on (A) 10μg/ml H/120 or (B) FnIII(6-10) for 60 minutes before addition of the mAbs K20, 12G10 or mAb13 for 30 minutes as indicated. Cells were then fixed and processed to visualise vinculin (green) and actin (red). The mean cell area ± s.d. (arbitrary units) was 1549.5 ± 822.0 for H/120 only (n=73), 1491.3 ± 713.2 for H/120 plus 12G10 (n= 83), 1485.8 ± 758.8 for H/120 plus K20 (n=87), 1164.7 ± 590.3 for H/120 plus mAb13 (n=101), 1151.1 ± 721.5 for FnIII(6-10) only (n=87), 1128.7 ± 643.8 for FnIII(6-10) plus 12G10 (n=84), 1129.1 ± 735.8 for FnIII(6-10) plus K20 (n=99), and 687.4 ± 487.3 for FnIII(6-10) plus mAb13 (n=90). Statistical differences were not detected (p>0.1; two tailed t-test) between the means of any of the groups (for comparisons made of cells spread on the same ligand) except for H/120 only vs. H/120 plus mAb13 (p<0.01) and FnIII(6-10) only vs. FnIII(6-10) plus mAb13 (p<0.01).