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. Author manuscript; available in PMC: 2012 Apr 16.
Published in final edited form as: J Biol Chem. 2005 Jan 4;280(11):10234–10243. doi: 10.1074/jbc.M411102200

Figure 6. Effect of 12G10 on soluble ligand binding to K562-α4 cells.

Figure 6

Binding of Oregon green-labelled VCAM-1-Fc to K562-α4 cells in suspension was detected by FACS. Cells were incubated in the absence of antibody (No mAb) or in the presence of 12G10 (activating anti-β1 mAb), K20 (non-functional anti-β1 mAb), HP2/1 (anti-functional anti-α4 mAb), 4B4 (anti-functional anti-β1 mAb) or EDTA. Mean Fluorescence Intensity (MFI) is shown for each condition (10000 events).