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HPLC/MS analysis of the spent medium of oral flora grown in salivary medium supplemented with and without 0.05% (w/v) sucrose. (A) TIC (left panel) and SIM (right panel, with deprotonated molecular ion 89 m/z) chromatograms: top, a standard lactic acid sample at 3.25 mM prepared in ddH2O; middle, spent medium with 0.05% sucrose; bottom, spent medium without sucrose. (B) ESI-MS of standard lactic acid (left) and corresponding peak in the spent medium with 0.05% sucrose (right).
