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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Sep 13;26(6):337–352. doi: 10.1111/j.2041-1014.2011.00622.x

Figure 5.

Figure 5

Co-cultivation of Pa060928 with salivary bacteria (S-mix) in the absence and presence of sucrose. (A) PCR-DGGE analysis of microbial profiles obtained from biofilm and planktonic portion of the co-culture after 24-hour co-incubation. S-mix/Pa060928 indicates co-culture of S-mix and Pa060928. The arrows indicate the DNA bands corresponding to Pa060928. Three biological replicates were performed and a representative gel image is shown. (B) Viability count of Pa060928 and total bacteria within planktonic and biofilm portions after 24-hour incubation in saliva medium. Three replicates were performed for each viability count assay. Average values + SD are plotted. (C) PCR-DGGE analysis of total bacterial profile (including planktonic and biofilm portion) after 24-hour co-cultivation in the absence and presence of 0.05% sucrose. Three biological replicates were performed and a representative gel image is shown. (D) Viability count of Pa060928 within the co-culture (combining planktonic and biofilm portion) after 24-hour incubation in the absence and presence of sucrose. Black bars represent Pa060928 in the co-culture without sucrose; open bars represent Pa060928 in the co-culture supplemented with 0.05% sucrose. Three replicates were performed for each viability count assay. Average values + SD are plotted.