Figure 4.

FIP200 binding site in Atg13 is encoded by exon 12. (A) Schematic representation of Atg13 splice variants (named A-G) amplified from DT40 cells. (B) The relative abundance of these splice variants was analyzed by qRT-PCR using splice variant-specific primer combinations (see Supplementary Material and Methods and Fig. S8A). (C) atg13^−/− cells were reconstituted with HA-tagged versions of splice variants A-G and lysates were subjected to anti-HA immunoprecipitation and analyzed for Atg13 and FIP200 by immunoblotting. Asterisk indicates an unspecific background band.