Figure 2.

Formation of a new ATG12 conjugate upon vaccinia virus infection. (A) Immunoblotting assay of wild-type, atg5^−/− and atg7^−/− MEF cell lines either untreated, or amino acid and serum deprived for 2 h with Hank’s media (starvation), or infected with vaccinia virus at a MOI of 3 and harvested 24 h post infection. Lysates were subjected to SDS-PAGE and separated proteins were immunoblotted using an anti-ATG12 (upper) or anti-actin (lower) antibody. The results are representative of three independent experiments. (B) Immunoblotting assay of wild-type cells showing the time course of the appearance of the novel protein band after vaccinia virus infection. Wild-type MEFs were infected with vaccinia virus at a MOI of 3, harvested at indicated time points, and analyzed with an anti-ATG12 (upper) or anti-actin (lower) antibody.