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The GFP-Atg8 protease protection assay. Cultures of vam3ts and atg1Δ cells expressing GFP-Atg8 grown to mid-log phase at 25°C were preincubated at 37°C for 30 min, and then shifted to starvation temperature at 37°C, for 1 h. Osmotically lysed cell extracts were analyzed for sensitivity to proteinase K (PK) in the presence or absence of 0.2% Triton X-100 (TX).
