Figure 4. p27^KIP1 protein, but not mRNA, is differential between birds at the onset of morphological divergence.

(A) Western blot analysis of p27 protein (lower doublet) relative to alpha-tubulin loading control (upper band) in a time course of microdissected samples from HH17-HH31 chicken and duck frontonasal prominences. (B) Levels of p27^KIP1 mRNA transcripts relative to GAPDH control in chicken and duck frontonasal prominences, as measured by qRT-PCR.