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. 2012 Apr 16;7(4):e35368. doi: 10.1371/journal.pone.0035368

Figure 2. Curcumin activates PKD1.

Figure 2

A). Effect of curcumin on PKD1 levels. C4-2 cells were treated with 20 µM curcumin. At varying time points, the cells were harvested, and the lysates were resolved on SDS-PAGE, transferred onto a PVDF membrane and probed for total PKD1. β-actin was used as an internal loading control. The band intensities were densitometrically analyzed, normalized to β-actin levels and graphed. Curcumin treatment resulted in no marked change in PKD1 expression at 1, 3 and 6 h. B). Effect of curcumin on activation of PKD1. For determining the expression of activated/phosphorylated PKD1, blots were probed with phospho PKD1, total PKD1 and β-actin antibodies. The pPKD1 band intensity was normalized to total PKD1 levels and graphed. Curcumin treatment induced PKD1 activation/phosphorylation by 1 h, while no apparent changes were observed in the expression of total PKD1. Representative blots of three experiments are shown in the figure. AU- arbitrary units.