PFGE and Southern hybridization of NC-MiCs. Uncut genomic DNA of
NC-MiCs 3a, 3b, 4, and 5 was run on 0.6% gel, 0.5×
Tris-acetate/EDTA buffer, at 1 V/cm, 192 h, transferred to
HybondN+ and probed with 32P-labeled E8 DNA.
NC-MiC3a and b migrated at a size of ≈1.7 Mb (arrowed), whereas
NC-MiCs 4 and 5 failed to enter the gel.