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. 1990 Dec 11;18(23):7077–7081. doi: 10.1093/nar/18.23.7077

Antigen expression from the ribosomal DNA repeat unit of Giardia intestinalis.

J A Upcroft 1, A Healey 1, R Mitchell 1, P F Boreham 1, P Upcroft 1
PMCID: PMC332772  PMID: 2263466

Abstract

The amitochondrial human intestinal parasite Giardia intestinalis is regarded to be the most ancient living example of single-celled eukaryotes and should display primitive features of pre-metazoan gene regulation. Characterization of E. coli clones which express Giardia antigens from plasmid vectors has revealed that an antigen is encoded by the rDNA repeat unit from the strand complementary to that encoding the rRNAs. The open reading frame (ORF) originates in the spacer region between the small (SS) and large (LS) subunit rRNA genes and terminates within the LS rRNA gene. The promoter region of this ORF has characteristics of both RNA polymerase (pol) II and pol III regulatory sequences, suggestive of gene regulation before these different promoter types evolved. The rDNA repeat unit is located on multiple chromosomal sites which are different in each isolate, although the electrophoretic karyotypes appear very stable in Giardia from both human and animal sources.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boothroyd J. C., Wang A., Campbell D. A., Wang C. C. An unusually compact ribosomal DNA repeat in the protozoan Giardia lamblia. Nucleic Acids Res. 1987 May 26;15(10):4065–4084. doi: 10.1093/nar/15.10.4065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Capon A. G., Upcroft J. A., Boreham P. F., Cottis L. E., Bundesen P. G. Similarities of Giardia antigens derived from human and animal sources. Int J Parasitol. 1989 Feb;19(1):91–98. doi: 10.1016/0020-7519(89)90026-x. [DOI] [PubMed] [Google Scholar]
  3. Cavalier-Smith T. The origin of eukaryotic and archaebacterial cells. Ann N Y Acad Sci. 1987;503:17–54. doi: 10.1111/j.1749-6632.1987.tb40596.x. [DOI] [PubMed] [Google Scholar]
  4. Comeau A. M., Miller S. I., Wirth D. F. Chromosome location of four genes in Leishmania. Mol Biochem Parasitol. 1986 Nov;21(2):161–169. doi: 10.1016/0166-6851(86)90019-8. [DOI] [PubMed] [Google Scholar]
  5. Crossley R., Holberton D. V. Characterization of proteins from the cytoskeleton of Giardia lamblia. J Cell Sci. 1983 Jan;59:81–103. doi: 10.1242/jcs.59.1.81. [DOI] [PubMed] [Google Scholar]
  6. Crossley R., Holberton D. Assembly of 2.5 nm filaments from giardin, a protein associated with cytoskeletal microtubules in Giardia. J Cell Sci. 1985 Oct;78:205–231. doi: 10.1242/jcs.78.1.205. [DOI] [PubMed] [Google Scholar]
  7. Crossley R., Marshall J., Clark J. T., Holberton D. V. Immunocytochemical differentiation of microtubules in the cytoskeleton of Giardia lamblia using monoclonal antibodies to alpha-tubulin and polyclonal antibodies to associated low molecular weight proteins. J Cell Sci. 1986 Feb;80:233–252. doi: 10.1242/jcs.80.1.233. [DOI] [PubMed] [Google Scholar]
  8. Edlind T. D., Chakraborty P. R. Unusual ribosomal RNA of the intestinal parasite Giardia lamblia. Nucleic Acids Res. 1987 Oct 12;15(19):7889–7901. doi: 10.1093/nar/15.19.7889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feagin J. E., Jasmer D. P., Stuart K. Apocytochrome b and other mitochondrial DNA sequences are differentially expressed during the life cycle of Trypanosoma brucei. Nucleic Acids Res. 1985 Jun 25;13(12):4577–4596. doi: 10.1093/nar/13.12.4577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  11. Gutell R. R., Fox G. E. A compilation of large subunit RNA sequences presented in a structural format. Nucleic Acids Res. 1988;16 (Suppl):r175–r269. doi: 10.1093/nar/16.suppl.r175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  13. Healey A., Mitchell R., Upcroft J. A., Boreham P. F., Upcroft P. Complete nucleotide sequence of the ribosomal RNA tandem repeat unit from Giardia intestinalis. Nucleic Acids Res. 1990 Jul 11;18(13):4006–4006. doi: 10.1093/nar/18.13.4006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ho Y. S., Howard A. J., Crapo J. D. Nucleotide sequence of a rat glutathione peroxidase cDNA. Nucleic Acids Res. 1988 Jun 10;16(11):5207–5207. doi: 10.1093/nar/16.11.5207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Holberton D., Baker D. A., Marshall J. Segmented alpha-helical coiled-coil structure of the protein giardin from the Giardia cytoskeleton. J Mol Biol. 1988 Dec 5;204(3):789–795. doi: 10.1016/0022-2836(88)90370-1. [DOI] [PubMed] [Google Scholar]
  16. Howell M. J., Hargreaves J. J. Cloning and expression of Taenia ovis antigens in Escherichia coli. Mol Biochem Parasitol. 1988 Feb;28(1):21–30. doi: 10.1016/0166-6851(88)90175-2. [DOI] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Long E. O., Dawid I. B. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. doi: 10.1146/annurev.bi.49.070180.003455. [DOI] [PubMed] [Google Scholar]
  19. Lyons K. M., Stein J. H., Smithies O. Length polymorphisms in human proline-rich protein genes generated by intragenic unequal crossing over. Genetics. 1988 Sep;120(1):267–278. doi: 10.1093/genetics/120.1.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murphy S., Moorefield B., Pieler T. Common mechanisms of promoter recognition by RNA polymerases II and III. Trends Genet. 1989 Apr;5(4):122–126. doi: 10.1016/0168-9525(89)90043-7. [DOI] [PubMed] [Google Scholar]
  21. Pritchard A. E., Venuti S. E., Ghalambor M. A., Sable C. L., Cummings D. J. An unusual region of Paramecium mitochondrial DNA containing chloroplast-like genes. Gene. 1989 May 15;78(1):121–134. doi: 10.1016/0378-1119(89)90320-x. [DOI] [PubMed] [Google Scholar]
  22. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  23. Sogin M. L., Gunderson J. H., Elwood H. J., Alonso R. A., Peattie D. A. Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia. Science. 1989 Jan 6;243(4887):75–77. doi: 10.1126/science.2911720. [DOI] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Upcroft J. A., Boreham P. F., Upcroft P. Geographic variation in Giardia karyotypes. Int J Parasitol. 1989 Aug;19(5):519–527. doi: 10.1016/0020-7519(89)90082-9. [DOI] [PubMed] [Google Scholar]
  27. Upcroft J. A., Capon A. G., Dharmkrong-At A., Healey A., Boreham P. F., Upcroft P. Giardia intestinalis antigens expressed in Escherichia coli. Mol Biochem Parasitol. 1987 Dec;26(3):267–276. doi: 10.1016/0166-6851(87)90079-x. [DOI] [PubMed] [Google Scholar]
  28. Upcroft P. Entrapment of recombinant plasmids in SeaPlaque agarose plugs and their rapid purification from recircularised vectors. Gene. 1988 May 30;65(2):319–323. doi: 10.1016/0378-1119(88)90469-6. [DOI] [PubMed] [Google Scholar]
  29. Van der Ploeg L. H., Cornelissen A. W., Barry J. D., Borst P. Chromosomes of kinetoplastida. EMBO J. 1984 Dec 20;3(13):3109–3115. doi: 10.1002/j.1460-2075.1984.tb02266.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zuker M. On finding all suboptimal foldings of an RNA molecule. Science. 1989 Apr 7;244(4900):48–52. doi: 10.1126/science.2468181. [DOI] [PubMed] [Google Scholar]

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