Figure 3.

Axin2 co-localizes with Irf6 in mouse palate epithelium. Coronal sections through mid- and anterior-palate regions of wild-type embryos pre- (E13, A-F; E14, G-L) and post-palate fusion (E15, M-O) were subject to immunofluorescent labeling with Axin2- and Irf6-specific polyclonal antibodies. Axin2 expression (green) is evident in palate and tooth epithelium and mesenchyme, in addition to tongue and oral epithelium and tongue musculature throughout palatogenesis (A, D, G, J, and M). Irf6 (pink) is expressed primarily in palate, tooth, oral, and tongue epithelium (B, E, H, K, and N). Merged images (yellow; C, F, I, L, and O) show co-localized expression of Axin2 and Irf6 in these epithelial cell layers critical to normal palate and tooth development.