(A) Cyanamide induced apoptosis in granulosa cells as detected by TUNEL assay
(B) The expression genes known to be markers of granulosa cell differentiation and follicular development, *; Significant differences were observed as compared with those in granulosa cells recovered from mice coinjected with eCG and cyanamide (P<0.05). Values are mean +/−SEM of three replicates. immature: immature mice before eCG priming, control: 48 hr after eCG priming, cyanamide: coinjection with eCG and 15 mg/kg of cyanamide for 48 hr