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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Clin Immunol. 2012 Mar 9;143(2):128–133. doi: 10.1016/j.clim.2012.02.006

Figure 1. PD-1−/− T cells are not resistant to anergy induction in vitro and in vivo.

Figure 1

A. Induction of naïve WT and PD-1−/− CD4+ T cell anergy by CD28 blockade was described in “Materials and Methods”. B. Induction of naïve WT and PD-1−/−CD4+ T cell anergy by ionomycin was described in “Materials and Methods”. C. Naïve CD4+KJ1-26+CD25− T cells from DO11.10 and DO11.10.PD-1−/− mice were adoptively transferred into BALB/c recipients, the mice were treated by either immunized or tolerized protocol, or left untreated. Ag-specific T cell expansion was detected by BrdU staining, and analyzed by flow cytometry. The figures are representative of at least four mice for each group. Data represent three or four independent experiments.