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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Feb 3;52(5):1135–1144. doi: 10.1016/j.yjmcc.2012.01.021

Figure 2.

Figure 2

NF-κB induction in MyD88−/− cardiomyocytes. Cfb RNA was quantified from WT and MyD88−/− cultured neonatal cardiomyocytes after 12 h treatment with LPS (A) or TNF-α (B). Expression was compared by ΔΔCT method and statistical analyses performed using One-way ANOVA and Bonferroni’s post-test. For testing role of MyD88 in NF-κB induction, cultured neonatal cardiomyocytes from WT (C) and MyD88−/− (C and D) mice were transfected with an NF-κB-luciferase reporter plasmid. After 48 h of transfection, cells were treated with 10 µg per ml LPS (C) or 4 µg per ml poly(I:C) (D) for indicated time. Controls (Ctr) were left untreated. Luciferase activity from a Renilla luciferase reporter was used as a control for transfection efficiency.