Figure 3.
Immune cell infiltration in infarcted myocardium. (A) Tissue sections from post-MI or sham operated (1 day) hearts from WT and MyD88−/− mice were labeled with neutrophil and granulocytes marker S100a9 (green fluorescence). Nuclei were stained with DAPI (Blue). Scale bars represent 50 µm. (B) Average number of infiltrating cells were counted in multiple sections from 3 hearts. MyD88−/− hearts had significantly less infiltration (unpaired t-test, ***P= 0.0008). (C) Myeloperoxidase (MPO) activity in sham operated or MI hearts (1-day post surgery) was performed as described in the text. MyD88−/− hearts showed significant reduction in MPO activity (One way ANOVA with Bonferroni’s post-test; n=3 each, *P≤ 0.01).
