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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Anal Biochem. 2012 Mar 3;424(2):178–183. doi: 10.1016/j.ab.2012.02.033

Fig. 2.

Fig. 2

Dequenching of BSA is specific to protease activity. (A) Dequenching was not observed when BSA-AF647 was incubated with a combination of pepsin and pepstatin, a pepsin inhibitor. (B) Gel electrophoresis (4–20% gradient, polyarcrylamide, SimplyBlue SafeStain) shows that the degradation of BSA (67 kDa) by pepsin is inhibited by pepstatin. Pepsin (35 kDa) is visible on the gel while pepstatin (0.7 kDa) and the degraded BSA fragments are too small to observe.