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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Anal Biochem. 2012 Mar 3;424(2):178–183. doi: 10.1016/j.ab.2012.02.033

Fig. 5.

Fig. 5

Two-color single particle tracking of BSA-AF647 and LAMP1-EYFP. (A) Snapshots illustrate the dequenching of BSA-AF647 (red) in a LAMP1-positive endo-lysosomal vesicle (green). Images were recorded at a rate of 0.5 Hz. (B) Intensity of the BSA-AF647 as a function of time. The horizontal bar under the intensity trace indicates the period of time during which BSA-AF647 was colocalized with a LAMP1-vesicle.