Figure 2.

Model validation of PLM-KO cardiomyocytes using data from Despa et al. [4-5]. A, ISO stimulation reduces [Na⁺]_i in resting WT, but not PLM-KO myocytes [4]. B, Cytosolic Ca²⁺ adaptation is uniquely present in the WT myocyte and coincides with the drop in intracellular Na⁺ [5]. C, Model Ca²⁺ transients from control (*), 2 min. early ISO stimulation (^†) and 30 min. steady-state ISO stimulation (^‡) are consistent with experimental findings [5]. D, Twitch Ca²⁺ amplitude decreases in the WT myocyte at steady-state, indicating Ca²⁺ adaptation [5]. E, ISO-stimulated SR load is larger in PLM-KO myocytes than WT myocytes [5]. F, ISO-stimulated SR loading is similar between model and experiment [5].