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. 2012 Jan 25;37(6):1323–1337. doi: 10.1038/npp.2011.317

Figure 6.

Figure 6

Effects of transnasally delivered NPS (14 nmol) 4 h after application on protein and mRNA levels in the prefrontal cortex and hippocampus of C57BL/6N mice. (a) Immunoblot analysis of hippocampal (Hc) lysate from C57BL/6N mice 4 h after intranasal NPS treatment. GluR1: t=1.192, df=8, p=0.2675; GluR2: t=0.657, df=8, p=0.5552; Glt-1: t=0.02711, df=8, p=0.9790; synapsin Ia–b/IIa: t=1.506, df=6, p=0.1828. (b) Real-time PCR analysis of Hc lysate from C57BL/6N mice 4 h after intranasal NPS treatment. GluR1: t=1.236, df=7, p=0.2562; GluR2: t=1.242, df=8, p=0.2493; synapsin I: t=0.3580, df=4, p=0.7385; synapsin II: t=1.243, df=7, p=0.2540. (c) Immunoblot analysis of prefrontal cortex (Pfc) lysate from C57BL/6N mice 4 h after intranasal NPS treatment. GluR1: t=0.1030, df=8, p=0.9205; GluR2: t=0.8469, df=8, p=0.4217; Glt-1: t=0.03901, df=8, p=0.9698; synapsin Ia–b/IIa: t=1.384, df=6, p=0.2038. (d) Real-time PCR analysis of Pfc lysate from C57BL/6N mice 4 h after intranasal NPS treatment. GluR1: t=0.7166, df=5, p=0.5057; GluR2: t=0.6839, df=8, p=0.5133; synapsin I: t=1.063, df=7, p=0.3230. Internal expression control: GAPDH (35 kDa in immunoblot excerpts). The blot excerpts show three representative adjacent bands of each group. The immunoblot data represent cumulated data from at least three independent experiments. C57BL/6N: n=5 for each group. Statistical analysis: Two-tailed unpaired t-test. tp<0.1, *p<0.05, **p<0.01. All data are shown as mean±SEM.