FIG. 1.
Cell viability as determined by MTT assay. (A) Wild-type p53-expressing A2780, A2780/CP70, OVCA 420, OVCA 429, and OVCA 433 cells. (B) P53-null (SKOV-3) and p53-mutated (OVCAR-3 and OVCA 432) cells. Cells were cotreated with the indicated concentrations of cisplatin with (filled symbols) or without (open symbols) 20μM sodium arsenite and incubated at 37°C (circles) or 39°C (triangles) for 1 h. Cells were then washed twice with PBS and refed with fresh media and incubated for 5 days at 37°C. MTT assay was performed on day 5. Data are expressed as percentage of untreated control and plotted as means ± SEM of at least four independent experiments each performed with triplicate wells. R2 values for the best fitting quadratic polynomial curves were all > 0.99.