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. 2012 Feb 13;127(1):139–149. doi: 10.1093/toxsci/kfs085

FIG. 2.

FIG. 2.

Cell viability and Western blot analysis of cells transfected with p53 siRNA. (A and C) Western blot analyses of A2780 and A2780/CP70 cells respectively each transfected with p53 siRNA, NSC, or mock. (B and D) Viability of respective A2780 and A2780/CP70 cells transfected with p53 siRNA, NSC, or mock. A2780 and A2780/CP70 cells were transfected with p53 smart pool siRNA, NSC, or mock. At 23 h after transfection, cells were treated with the indicated concentrations of cisplatin with (•) or without (o) 20μM sodium arsenite for 1 h at 37°C. Protein lysates for Western blot analyses were prepared at 24 and 48 h after transfection. MTT assay was performed 5 days after treatment. Data in panels A and C are representative of three independent experiments. Data in panels B and D are means ± SD from three independent experiments.