FIG. 3.

Cisplatin concentration–dependent induction of p53, p53Ser15P, XPC, and DDB2. Cells were treated with the indicated concentrations of cisplatin (CP) at 37°C for 1 h. After treatment, cells were washed twice with PBS and incubated in drug-free media for 24 h, and protein lysates were then prepared and analyzed by Western blot as detailed in the Materials and Methods section. ß-actin is the loading control. Blots are representative of three independent experiments.