FIG. 5.
Cellular platinum accumulation and effect of sodium arsenite and hyperthermia. (A) Cisplatin concentration–dependent accumulation of platinum in A2780 (▴), A2780/CP70 (▪), and SKOV-3 (•) cells. The regression equation for A2780 cells is: y = 0.68 + 0.74x; A2780/CP70 cells, y = 0.55 + 0.30x; SKOV-3 cells, y = 0.11 + 0.75x; R2 = 0.95 for the overall fit to the data. (B) Effect of sodium arsenite and hyperthermia on cellular platinum accumulation. A2780, A2780/CP70, and SKOV-3 cells were treated with the indicated cisplatin concentrations for 1 h at 37°C (A) or with 40μM cisplatin ± 20μM sodium arsenite at 37°C or 39°C for 1 h (B). Cells were harvested immediately after treatment for total cellular platinum determination. Data are plotted as means ± SD of three independent experiments. p < 0.05. *compared with CP37, # compared with CPA 37, and f compared with CP39.